Figure 5.

(A) Alignment of amino acid sequences of PIG-W homologs of human, rat, S. cerevisiae, and S. pombe. Black and gray boxes indicate identical and conserved amino acids, respectively. Six regions highly conserved in four species are indicated by broken underlines. Positions of 13 predicted transmembrane domains of S. pombe PIG-W are indicated by numbered underlines. An arrowhead indicates leucine changed to a stop codon in 1D10 mutant. (B) Hydropathic profile of human PIG-W. Hydrophobicity was calculated with a window length of 17 (Kyte and Doolittle, 1982). Positions of 13 predicted transmembrane domains are indicated by numbered thick bars.