Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Oct;14(10):4285–4295. doi: 10.1091/mbc.E03-03-0193

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The American Society for Cell Biology

PMC Copyright notice

Figure 8. — The PIG-W gene encodes acyltransferase. (A) FLAG-PIG-W-FLAG-HAT (lanes 1 and 4), FLAG-PIG-W-GST (lanes 2 and 5), and PIG-LGST-FLAG (lanes 3 and 6) were isolated by two-step affinity purification from the lysates of transfected JY25 cells. M, molecular size markers; lanes 1–3, silver staining; lanes 4–6, Western blotting with anti-FLAG antibody. (B) The purified FLAG-PIG-W-GST (lanes 1 and 3) and PIG-L-GST-FLAG (lane 2) proteins were incubated with [³H]palmitoyl-CoA in the presence (lanes 1 and 2) or absence (lane 3) of GlcN-PI(C8). After 10-min-incubation, lipids were analyzed by TLC with a solvent system of chloroform/methanol/0.25% KCl (55:45:10). GlcN-acylPI(C8) generated (asterisk) was treated overnight with GPI-PLD (lane 4), buffer for GPI-PLD (lane 5), PI-PLC (lane 6), or buffer for PI-PLC (lane 7), extracted again, and analyzed by TLC.