Figure 9.

Membrane orientation of the N and C termini of PIG-W. Rat PIG-W tagged at the C termini (PIG-W-FLAG) (a–f) or the N terminus (FLAG-PIG-W) (g–l) were expressed in CHO cells. After permeabilization of both the plasma membrane and the ER membrane (a–c and g–i), or selective permeabilization of the plasma membrane (d–f and j–k), cells were stained for tagged PIG-W by anti-FLAG antibody (b, e, h, and k), for an endogenous ER luminal protein BiP (a, d, g, and j) and a cytosolic protein Hsc70 (c, f, I, and l).