Tunicamycin induces Swe1-dependent delay at G2/M phase of the cell cycle and Swe1-independent cell survival pathway (A) Asynchronous log-phase cultures of wild-type cells, mpk1 mutants, and swe1 mutants (strains K1257, RG00993, and RG01238) were treated with tunicamycin or tunicamycin plus FK506 for the indicated periods of time, fixed, stained with DAPI, and analyzed by fluorescence microscopy. The percentage of cells in the population containing two or more nuclei is plotted. (B) Dead cells in the cultures used in A were identified by light microscopy after staining with methylene blue, and the percentage of surviving cells in the population is plotted.