Figure 4.

Phosphorylation of FAK by α5β1 binding to substrate fibronectin in HOS cells. Similar to Figure 3 except experiments shown are for HOS cells. (A) A representative blot. (B) The quantified combined data from three independent experiments FAK tyrosine phosphorylation. Error bars, SEM for n = 3 separate experiments. Linear regression: pY397 R² = 0.94, p = 0.0059; pY407 R² = 0.74, p = 0.06; pY577 R² = 0.81, p = 0.0382; pY861 R² = 0.97, p = 0.0027.