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(Upper) Thermodynamic cycles and free-energy diagram for the oxidized (OX) and reduced (RED) states of unfolded (U) and folded (F) forms of azurin. The unfolded copper sites can have different conformations (U, U′) with different relative stabilities in the oxidized and reduced states. (Lower) GuHCl denaturation curves and unfolding isotherms for oxidized (CuII) and reduced (CuI) Pseudomonas aeruginosa azurin (22°C).
