Figure 2.

(A) C_α trace of the protein structure of subtilisin Carlsberg in anhydrous dioxane (black lines) with bound water (gray balls) and dioxane (balls-and-sticks: C atoms in black and O atoms in gray) molecules. (B) Superimposition of the C_α traces of subtilisin in water (thin gray lines) and in anhydrous dioxane (thicker black lines). The rms shifts of the C_α atoms and of the main-chain atoms are 0.31 Å and 0.33 Å, respectively. Ser-159 and Gly-160 are not included in the model of subtilisin in dioxane because of uncertainties in their location; the C_α atoms of Asn-158 and Ser-161 are shown as balls (upper right).