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(A) Refined crystallographic B factors (residue average) of subtilisin Carlsberg in dioxane (solid line), water (dashed line), and acetonitrile (dotted line). The difference in the exposed surface areas of subtilisin between dioxane and water (B) and between acetonitrile and water (C). As in Fig. 2, Ser-159 and Gly-160 are left out in A and B.
