Figure 2.

Comparison of cross-sectional OCT images from a single Xenopus heart with corresponding histology. Standard histological preparation was performed and stained with hematoxylin/eosin. Correlation between OCT images and histology is strong. (A and B) Image and corresponding histology of the major arteries of the ventral aorta, anterior to bifurcation. (C and D) Atria within the pericardial sac. (E and F) Cross-section of ventricle showing trabeculae carneae network and papillary muscles within the chamber. Note the preparation artifacts frequently present in histology, such as the distorted outer membrane and chamber displacement and the ability of OCT to acquire optical histology while preserving in vivo orientation. a, atrium; aa, arteries with lumens; v, ventricle.