TABLE 3.
Commercial assays used to identify E. histolytica
| Assay | Sensitivity (%) | Specificity (%) | Manufacturer |
|---|---|---|---|
| Antigen detection | |||
| TechLab E. histolytica II | 100a | ∼95a | TechLab, Blacksburg, Va. |
| TechLab Entamoeba test | 80b-95c | 99b-93c | TechLab, Blacksburg, Va. |
| ProSpecT Entamoeba histolytica microplate assay | 90.3d | 97.7d | Alexon-Trend Inc., Ramsey, Minn. |
| Entamoeba CELISA-PATH | KPo (94) | KP (100) | Cellabs Pty Ltd., Brookvale, Australia |
| Entamoeba-CELISA-Screen | KP (87.7) | KP (98.3) | Cellabs Pty Ltd., Brookvale, Australia |
| Wampole E. histolytica Test | KP (94.7)e | KP | Wampole Laboratories, Cranbury, N.J. |
| Merlin Optimun S ELISA | 100f | Merlin Diagnostika, Bernheim-Hersel, Germany | |
| Triage parasite panel | 68.3g | 100g | BIOSITE Diagnostics, San Diego, Calif. |
| 83.3h | 100h | ||
| 96i | 99.1i | ||
| Amibiase Ag EIA | NPp | NP | Biotrin Int., Dublin, Ireland |
| Antibody detection | |||
| IHA Cellognost Amoebiasis | 72.2j-100k | 99.1j-90.9k | Behring Diagnostics, Marburg, Germany |
| Amibiasis Serology Microwell EIA | 92.5l | 91.3l | LMD Laboratories Inc., Carlsbad Calif. |
| BLA-Bichrolatex-Amibe | 98.3m | 96.1m | Fumouze Diagnostics, Levallois-Perret, France |
| IHA | 93.4m | 97.5m | Fumouze Diagnostics, Levallois-Perret, France |
| The Melotest Amoebiasis EIA | NAn | NAn | Melotec, S.A., Barcelona, Spain |
a
Compared to culture and isoenzyme analysis (78).
b
Compared to culture (85).
c
Compared to zymodeme analysis (85).
d
Compared to microscopy (99).
e
Correlation to zymodeme analysis.
f
Correlation of TechLab E. histolytica for detection of E. histolytica, but not E. dispar (161).
g
Compared to ProSpecT ELISA (160).
h
Compared to microscopy (160).
i
Compared to O&P and permanent stains (67).
j
Reference 91.
k
Reference 161 (with use of E. histolytica antigen detection as the reference standard).
l
Reference 204.
m
Reference 180.
n
Reference 182. NA, not available.
o
KP, kit prospectus.
p
NP, not published data.