Table 1.
Interaction between HLF and Arnt as revealed by the yeast two-hybrid system
| GAL-DBD fusions | GAL activation domain fusions
|
|||
|---|---|---|---|---|
| GAD424 | Arnt | HIF1α | HLF | |
| pGBT9 | <0.02* | <0.02 | <0.02 | <0.02 |
| Arnt | < 0.02 | 0.066 ± 0.016 | 1.71 ± 0.24 | 1.61 ± 0.1 |
| HIF1α | < 0.02 | 14.5 ± 5.9 | < 0.02 | < 0.02 |
| HLF | < 0.02 | 13.2 ± 3.0 | < 0.02 | < 0.02 |
Yeast strain SFY526 containing lacZ under the control of the GAL1 promoter was cotransfected with the indicated plasmids, and β-galactosidase activities were determined.
*
β-galactosidase activity (Miller units).