Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Sep;174(18):5985–5987. doi: 10.1128/jb.174.18.5985-5987.1992

An ordered clone bank for chromosome I of Saccharomyces cerevisiae.

S Tanaka 1, A Yoshikawa 1, K Isono 1
PMCID: PMC207138  PMID: 1522073

Abstract

Chromosome I of Saccharomyces cerevisiae DC5 rho 0 was dissected into segments with an average size of 14.0 kb and cloned into lambda phage vectors. The physical maps of the resultant clones, totaling 205.9 kb, were used to construct an ordered clone bank of this chromosome.

Full text

PDF
5985

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carle G. F., Olson M. V. An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756–3760. doi: 10.1073/pnas.82.11.3756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carle G. F., Olson M. V. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. doi: 10.1093/nar/12.14.5647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chan C. S., Tye B. K. Organization of DNA sequences and replication origins at yeast telomeres. Cell. 1983 Jun;33(2):563–573. doi: 10.1016/0092-8674(83)90437-3. [DOI] [PubMed] [Google Scholar]
  4. Coleman K. G., Steensma H. Y., Kaback D. B., Pringle J. R. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome. Mol Cell Biol. 1986 Dec;6(12):4516–4525. doi: 10.1128/mcb.6.12.4516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davies C. J., Hutchison C. A., 3rd A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I. Nucleic Acids Res. 1991 Oct 25;19(20):5731–5738. doi: 10.1093/nar/19.20.5731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diehl B. E., Pringle J. R. Molecular analysis of Saccharomyces cerevisiae chromosome I: identification of additional transcribed regions and demonstration that some encode essential functions. Genetics. 1991 Feb;127(2):287–298. doi: 10.1093/genetics/127.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dumont M. E., Ernst J. F., Hampsey D. M., Sherman F. Identification and sequence of the gene encoding cytochrome c heme lyase in the yeast Saccharomyces cerevisiae. EMBO J. 1987 Jan;6(1):235–241. doi: 10.1002/j.1460-2075.1987.tb04744.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaback D. B., Steensma H. Y., de Jonge P. Enhanced meiotic recombination on the smallest chromosome of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1989 May;86(10):3694–3698. doi: 10.1073/pnas.86.10.3694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  10. McNally T., Purvis I. J., Fothergill-Gilmore L. A., Brown A. J. The yeast pyruvate kinase gene does not contain a string of non-preferred codons: revised nucleotide sequence. FEBS Lett. 1989 Apr 24;247(2):312–316. doi: 10.1016/0014-5793(89)81359-6. [DOI] [PubMed] [Google Scholar]
  11. Miyamoto S., Ohya Y., Ohsumi Y., Anraku Y. Nucleotide sequence of the CLS4 (CDC24) gene of Saccharomyces cerevisiae. Gene. 1987;54(1):125–132. doi: 10.1016/0378-1119(87)90354-4. [DOI] [PubMed] [Google Scholar]
  12. Mortimer R. K., Schild D., Contopoulou C. R., Kans J. A. Genetic map of Saccharomyces cerevisiae, edition 10. Yeast. 1989 Sep-Oct;5(5):321–403. doi: 10.1002/yea.320050503. [DOI] [PubMed] [Google Scholar]
  13. Nash R., Tokiwa G., Anand S., Erickson K., Futcher A. B. The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog. EMBO J. 1988 Dec 20;7(13):4335–4346. doi: 10.1002/j.1460-2075.1988.tb03332.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  15. Schweitzer B., Philippsen P. CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain. Yeast. 1991 Apr;7(3):265–273. doi: 10.1002/yea.320070308. [DOI] [PubMed] [Google Scholar]
  16. Steensma H. Y., Crowley J. C., Kaback D. B. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and analysis of the CEN1-ADE1-CDC15 region. Mol Cell Biol. 1987 Jan;7(1):410–419. doi: 10.1128/mcb.7.1.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tanaka S., Isono K. Physical dissection and characterization of chromosomes V and VIII of Saccharomyces cerevisiae. Nucleic Acids Res. 1992 Jun 25;20(12):3011–3020. doi: 10.1093/nar/20.12.3011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vollrath D., Davis R. W., Connelly C., Hieter P. Physical mapping of large DNA by chromosome fragmentation. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6027–6031. doi: 10.1073/pnas.85.16.6027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Whyte W., Keopp L. H., Lamb J., Crowley J. C., Kaback D. B. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation, characterization and regulation of the SPO7 sporulation gene. Gene. 1990 Oct 30;95(1):65–72. doi: 10.1016/0378-1119(90)90414-m. [DOI] [PubMed] [Google Scholar]
  20. Wickner R. B. Host function of MAK16: G1 arrest by a mak16 mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6007–6011. doi: 10.1073/pnas.85.16.6007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wickner R. B., Koh T. J., Crowley J. C., O'Neil J., Kaback D. B. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation of the MAK16 gene and analysis of an adjacent gene essential for growth at low temperatures. Yeast. 1987 Mar;3(1):51–57. doi: 10.1002/yea.320030108. [DOI] [PubMed] [Google Scholar]
  22. Yoshikawa A., Isono K. Chromosome III of Saccharomyces cerevisiae: an ordered clone bank, a detailed restriction map and analysis of transcripts suggest the presence of 160 genes. Yeast. 1990 Sep-Oct;6(5):383–401. doi: 10.1002/yea.320060504. [DOI] [PubMed] [Google Scholar]
  23. Yoshikawa A., Isono K. Construction of an ordered clone bank and systematic analysis of the whole transcripts of chromosome VI of Saccharomyces cerevisiae. Nucleic Acids Res. 1991 Mar 25;19(6):1189–1195. doi: 10.1093/nar/19.6.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zakian V. A., Blanton H. M. Distribution of telomere-associated sequences on natural chromosomes in Saccharomyces cerevisiae. Mol Cell Biol. 1988 May;8(5):2257–2260. doi: 10.1128/mcb.8.5.2257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. de Steensma H. Y., de Jonge P., Kaptein A., Kaback D. B. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: localization of a repeated sequence containing an acid phosphatase gene near a telomere of chromosome I and chromosome VIII. Curr Genet. 1989 Sep;16(3):131–137. doi: 10.1007/BF00391468. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES