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. 1997 Apr 29;94(9):4289–4294. doi: 10.1073/pnas.94.9.4289

Figure 1.

Figure 1

Analysis of Ire1p interaction with Gcn5p and mapping of interaction domains. (a) LexA fusion proteins of the cytoplasmic domain and different subdomains within the cytoplasmic domain of Ire1p (bait) were tested for interaction with either the original clone B42–HA–Gcn5p (amino acids 1–342) or with B42–HA–Gcn5 BD (amino acids 349–439). Transformants harboring IRE1 and GCN5 fusions were patched onto His−Trp− and replica-plated onto His−Trp−Leu− plates containing either glucose or galactose. (b) Diagrammatic representation of the IRE1 deletions and their in vivo genetic and physical interaction activities. One-letter abbreviations for amino acids are used. WC, wild-type cytoplasmic/nucleoplasmic domain; MC, K702A mutant cytoplasmic/nucleoplasmic domain; NK, N-linker plus kinase domain; WK, wild-type kinase domain; MK, K702A mutant kinase domain; KC, kinase plus C-terminal tail; CT, C-terminal tail; ND, not detected.