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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Mar 16;1771(9):1132–1139. doi: 10.1016/j.bbalip.2007.03.002

Fig. 1.

Fig. 1

Micellar cholesterol binding to BBMV isolated from the small intestines of wild type mice. BBMV associated with 10 µg of protein from two wild type mice were incubated with various concentrations of bile salt mixed micelles containing [14C]cholesterol for 30 min at 4°C. Panel A shows concentration-dependent cholesterol binding to BBMV from wild type mice. The data represent representative of three independent experiments using BBMV preparations from different animals. Each data point represents the combined BBMV from 2 animals. Panel B shows Scatchard analysis of the binding data. Shown in the inset are linear representations of the high and low components of the binding curves with the estimated binding affinities (KD).