Fig. 2.

Cholesterol binding to BBMV from wild type mice. Panel A shows cholesterol binding to BBMV isolated from wild type mice and wild type mice fed ezetimibe as estimated by Scatchard analysis. Scatchard curves for cholesterol binding to wild type BBMV in the presence of ezetimibe (20 µM) or anti-mouse SR-BI neutralizing antibody are shown as dashed lines in panels B and C. Cholesterol binding to BBMV prepared from wild type (WT) mice is displayed in each of the figures as solid lines. Each data point represents 10 µg of protein from BBMV combined from two mice and representative of 3 or 4 repeats each with fresh BBMV preparations from additional animals.