FIGURE 2.

Phase diagrams of NCPs precipitated with magnesium (a) and spermidine (b) in the presence of 10 mM Tris-HCl. They display as a function of C_ap (mM) = 0.799C_NCPs (mg mL⁻¹). The positive charge amounts carried by magnesium or spermidine are plotted as a function of the negative charge amounts available on the NCPs. The equations Z_multiC_multi = C_ap are indicated by continuous lines. Dashed lines serve as a guide for the eye. Precipitation (▴) and redissolution (♦) points obtained from the precipitation curves are indicated. Above 60 mg mL⁻¹ (C_ap = 47.8 mM), the solution state was determined by visual inspection before centrifugation and was either turbid (•) or transparent (×).