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CD spectra of recombinant porcine amelogenins in (A) Tris-HCl buffer at pH = 5.8 at 25°C. The concentrations of the protein used are [rP172] = 5 μM; [rP148] = 3 μM; [25 C-term] = 34.5 μM; and [poly(proline)] = 6 μM. (B) Tris-HCl buffer containing 8 M guanidine hydrochloride; (C) in Tris-HCl buffer containing 2 M CaCl2.
