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. 1997 Apr 29;94(9):4324–4329. doi: 10.1073/pnas.94.9.4324

Figure 1.

Figure 1

S1 nuclease protection of shorter RNAs associated with stalled transcription complexes. (A) Schematic diagram of RNAs synthesized under limiting nucleotide concentrations in isolated mitochondria and S1 nuclease protection of the resulting RNA species. (B and C) S1 nuclease protection of labeled RNAs with unlabeled ssDNA corresponding to edited coI sequence (probe 1). (B) Lane 1, edited coI control transcript; lanes 2–5, RNAs synthesized in isolated mitochondria during a 3-min pulse labeling in the presence of limiting (radiolabeled) ATP, CTP, GTP, or UTP. (C) Lane 1, edited coI control transcript; lanes 2–5, RNAs synthesized in isolated mitochondria. Lanes: 2, 3-min pulse labeling, limiting GTP; 3, 15-min pulse labeling, limiting GTP; 4, 3-min pulse labeling, limiting GTP, followed by a 12 min chase with 100 μM GTP; 5, 3-min pulse labeling, limiting GTP, followed by a 12 min chase with 100 μM UTP.