Figure 3.

Size determination of ERF-1. (A) ERF-1 complex was resolved by gel-shift analysis and the gel was subjected to UV crosslinking. The bound complex was excised and resolved on SDS/PAGE as shown. The location of molecular weight markers (kDa) is shown to the left of the gel lane. (B) Purified extract was electrophoresed on 8% SDS/PAGE and sectioned into 12 gel slices. The approximate location of gel slices 1–12 are shown to the right of the gel lane. Molecular weight markers (kDa) are listed to the left of the lane labeled markers. (C) Gel shift analysis of proteins renatured from gel slices in B. Fraction number 7 demonstrated ERF-1 activity.