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In vitro synthesized ERF-1. (A) In vitro transcription/translation from the ERF-1 cDNA using T3 polymerase (sense) (lane 1) or T7 polymerase (antisense) (lane 3). In vitro synthesized ERF-1 was immunoprecipitated using AP2 polyclonal antisera (lane 2). (B) Gel shift analysis of in vitro synthesized ERF-1 (lanes 2–13) with mutant competitors compared with MCF7 nuclear extract (lane 1). In vitro ERF-1 complex supershifted with AP2 antisera (lane 13).
