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. 1997 Apr 29;94(9):4354–4359. doi: 10.1073/pnas.94.9.4354

Figure 2.

Figure 2

Identification of the protein required for GluR-B RNA editing in HeLa cells. (A) Lane 1: UV crosslinking of phenyl superose column fractions. GluR-B RNA labeled at the editing site was used in UV crosslinking experiments to detect proteins in the phenyl superose column fractions that bind to GluR-B RNA. The proteins were resolved by SDS/PAGE, and a 90-kDa crosslinked protein is indicated. Lane 2: Silver-stained SDS/PAGE of the most active fractions. Lane 3: Western blot of the flow-through. Lane 4: Western blot of the most active fraction. Lane 5: Western blot of recombinant rRED1 protein containing one ≈5 kDa His-tag at its N terminus. Western blot analysis was performed with anti-rat RED1 polyclonal antibodies. (B) The most active fraction was resolved on a two-dimensional gel [first dimension: isoelectric focusing pH 3–10; second dimension: SDS/PAGE (10% gel)] and by Western blot analysis with anti-rat RED1 polyclonal antibodies as above.