Figure 3.

Editing kinetics of the ds adenosine deaminases. (A) GluR-B Q/R site RNA editing by recombinant hRED1. The kinetics of the deaminase reactions were studied under single turnover reaction conditions ([protein] ≫ [GluR-B RNA]). The reaction was sampled at 0, 1, 3, 5, 10, 20, 30, 45, 60, and 90 min (lanes 1–10, respectively). For comparison, a parallel experiment with recombinant rRED1 is also shown. (B) Substrate selectivity of the ds adenosine deaminases. GluR-B RNA editing and dsRNA deamination activities were compared under single turnover reaction conditions. Increasing amounts of purified HeLa cell GluR-B activity (panel 1), recombinant hRED1 protein (panel 2), recombinant rRED1 protein (panel 3) and recombinant dsRAD protein (panel 4) were added to the reaction. The percentage of edited RNA was determined by thin layer chromatography and quantitated by the Fuji imaging system.