Figure 1.

Outline of bDNA assay. Short label and capture target probes are designed for a specific target sequence and hybridized with the target on a plate coated with oligonucleotides complementary to the capture target probes. The bDNA amplifier is then added and hybridized, followed by the AP probe, a short alkaline phosphatase-labeled oligonucleotide complementary to a triplicate repeat sequence on the bDNA branches. In the final step, a luminescent substrate of alkaline phosphatase, dioxetane, is added, and the emitted light is measured in a luminometer.