Table 1.
Oligonucleotide primers used in this study
| HDKUa | 5′ gCTggTTAAATAATAgTCgTTACTCAATTATTCTggATgggATTTgATAggCTggAgCTgCTTC 3′ |
| HDKLa | 5′ CAAAACggTTAACTACCgCTATTTCTTTACggCATCATCTTTCTgATATggATCCgTCgACCTgC 3′ |
| HumKUa | 5′ gCTTCCCTTCTCCTgCggCggATTATgTTgAAAgCCgAATTTCTCTTgAggCTggAgCTgCTTC 3′ |
| HumKLa | 5′ gCACCAgCACTTTgCAgCTTAAATgACCggACAATCATCAAACTCTggATCCgTCgACCTgC 3′ |
| Cam Tn9 Ua | 5′ CCgTTgATATATCCCAATggCATCgTAAAgAACATTTTgAggCTggAgCTgCTTC 3′ |
| Cam Tn9 La | 5′ ATTCATCAggCgggCAAgAATgTgAATAAAggCCggATCCgTCgACCTgC 3′ |
| Hole Upb | 5′ gCTgAAgAATCTggCTAAAC 3′ |
| Hole lowb,c | 5′ TTAAgTTTgggCTCgTAAg 3′ |
| HumD upperb | 5′ AAgCCgAATTTCTCTTgATC 3′ |
| HumD lowerb,c | 5′ gACAATCATCAAACTCTCCAC 3′ |
| Hot upperb | 5′ ggATTTgATATgTACgATTgg 3′ |
| Hot lowerb,c | 5′ ggCAACTggAggCTTAAC 3′ |
| Slowd,e | 5′ gTCTCCAgATCCTCCTTgC 3′ |
| Supd | 5′ ggTTTAATCACCggCTTAC 3′ |
a
Primers used to create deletions of the P1 genes hot (HDKU and HDKL), humD (HumKU and HumKL), and Tn9kan (Cam Tn9 U and Cam Tn9 L) (see text).
b
Gene-specific primers for genotyping and QPCR of indicated genes.
c
Gene-specific primers used to prepare cDNA.
d
Primers used for QPCR of gene 16.
e
Primer used for gene 16 cDNA preparation.