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. Author manuscript; available in PMC: 2007 Nov 9.
Published in final edited form as: Nature. 2001 Apr 19;410(6831):948–952. doi: 10.1038/35073616

Figure 3.

Figure 3

Robo is involved in mediating Slit inhibition of chemotaxis. a, The effect of Slit is inhibited by RoboN. Chemotaxis was analysed by using rat lymph node cells in transwell assays. SLIT2 (SL) and RoboN (RN) were added to the upper well and SDF-1α was added to the lower well. b, Expression of CXCR4 and Robo in HEK cells can reconstitute Slit inhibition of SDF-1α-induced chemotaxis. HEK migration was measured with the transfilter assay in microchemotaxis chambers using HEK cells expressing CXCR4, or both CXCR4 and rat Robo1 in the presence of different concentrations of SDF-1α. Control vehicle or 100 pM of purified Slit protein was added. Squares, migration of HEK cells expressing CXCR4 in response to SDF-1; diamonds, migration of HEK cells expressing CXCR4 in response to SDF-1α in the presence of Slit; circles, migration of HEK cells expressing Robo and CXCR4 in response to SDF-1α; triangles, migration of HEK cells expressing Robo and CXCR4 in response to SDF-1α in the presence of Slit.