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. Author manuscript; available in PMC: 2007 Nov 9.
Published in final edited form as: J Biol Chem. 2006 Jun 16;281(33):23302–23306. doi: 10.1074/jbc.C600143200

FIGURE 1. Stabilizing the tau stem loop in vitro.

FIGURE 1

A, several base changes predicted to stabilize the tau mRNA stem loop in vitro were made in both the WT and DDPAC (+14) stem loops. B, UV melting curves were generated for all 6 stem loop variants: DDPAC (○), DD-10C (△), DD-I17T (◊) WT (●), WT-10C (▲), and WT-I17T (♦). C, melting temperatures (Table 1) derived from the melting curves are plotted here of increasing stability. These nucleotide changes enhanced the thermal stability of both the WT and +14 mutated stem loops.