TABLE 1.
Stem loop variant | Predicted ΔGa | Measured Tm |
---|---|---|
kcal/mol | °C | |
DDPAC | −6.1 | 53.9 ± 0.6 |
DD-10C | −7.3 | 56.2 ± 1.3 |
DD-I17T | −11.4 | 59.6 ± 0.6 |
WT | −8.2 | 68.0 ± 1.1 |
WT-10C | −9.4 | 71.7 ± 1.5 |
WT-I17T | −13.5 | 73.0 ± 2.2 |
Stem loop variant | Predicted ΔGa | Measured Tm |
---|---|---|
kcal/mol | °C | |
DDPAC | −6.1 | 53.9 ± 0.6 |
DD-10C | −7.3 | 56.2 ± 1.3 |
DD-I17T | −11.4 | 59.6 ± 0.6 |
WT | −8.2 | 68.0 ± 1.1 |
WT-10C | −9.4 | 71.7 ± 1.5 |
WT-I17T | −13.5 | 73.0 ± 2.2 |