TABLE 1.

Relative stabilities of stem loop variants

Stem loop variant	Predicted ΔGa	Measured Tm
	kcal/mol	°C
DDPAC	−6.1	53.9 ± 0.6
DD-10C	−7.3	56.2 ± 1.3
DD-I17T	−11.4	59.6 ± 0.6
WT	−8.2	68.0 ± 1.1
WT-10C	−9.4	71.7 ± 1.5
WT-I17T	−13.5	73.0 ± 2.2

^aPredicted stabilities were calculated with MFOLD (22,23) for each of the stem loop variants on the identical sequences used in the in vitro melting experiments.