Table 2.
Genomic organization of P2Y12-like GPCR. n.a. not analyzed because of lack of mRNA information
| Member | Number of introns in the 5′ non-coding region | Number of introns in the coding region | Approx. size of genomic regiona |
|---|---|---|---|
| P2Y12 | |||
| Human | 1–2 | − | 47 kbp (2 different transcript starts) |
| Mouse | 4 | − | 46.5 kbp |
| Chicken | n.a. | −b | n.a. (>1 kbp) |
| African clawed frog | − | 1 (N terminus) | 4 kbp |
| Zebrafish | n.a. | −b | n.a. (>1 kbp) |
| Stickleback | 1 | 1 (TMD2) | 2 kbp |
| Pufferfish | n.a. | 1 (TMD2) | >2 kbp |
| P2Y13 | |||
| Human | − | 1 (rare transcript, N terminus, NM_176894) | 3.2 kp (2 different transcript starts) |
| Mouse | 1 | − | 3 kbp |
| Chicken | n.a. | −b | n.a. (>1 kbp) |
| African clawed frog | − | 1 (N terminus) | 14–19 kbp |
| P2Y14 | |||
| Human | 2 | − | 66 kbp (2 different transcript starts) |
| Mouse | 1–2 | − | 16 kbp |
| Chicken | n.a. | −b | n.a. (>1 kbp) |
| GPR87 | |||
| Human | 1 | 1 (N terminus) | 22.7 kbp |
| Mouse | 1 | 1 (N terminus) | 16 kbp |
| Chicken | 2 | 1 (N terminus) | 9.5 kbp |
| African clawed frog | n.a. | −b | n.a. (>1 kbp) |
| P2Y14/GPR87 | |||
| Zebrafish | n.a. | −b | n.a. (>1 kbp) |
| Pufferfish | n.a. | −b | n.a. (>1 kbp) |
| GPR171 | |||
| Human | 2 | − | 5 kbp |
| Mouse | 1 | − | 4.5 kbp |
| Chicken | 1 | − | 2.3 kbp |
| Zebrafish | n.a. | −b | n.a. (>1 kbp) |
| GPR82 | |||
| Human | 2 | − | 4 kbp |
| Mouse | 2–3 | − | 6 kbp |
| Chicken | n.a. | −b | n.a. (>1 kbp) |
| African clawed frog | n.a. | −b | n.a. (>1 kbp) |
| Zebrafish | n.a. | −b | n.a. (>1 kbp) |
| GPR34 | |||
| Human | 3–4 | 1 cryptic (N terminus) | 8.2 kbp |
| Mouse | 3–4 | − | 9.1 kbp |
| Chicken | 1 | − | 2.5 kbp |
| African clawed frog | 2 | − | 5.2 kbp |
| Zebrafish | |||
| Type 1 | 1 | − | 7.3 kbp |
| Type 2 | − | − | 1.2 kbp |
| Pufferfish | n.a. | −b | n.a. (>1 kbp) |
Except for GPR87, human P2Y12-like receptor members contain no intron in the coding region. However, this intronless gene structure is not well preserved in all vertebrates. The table summarizes the number of introns within the 5′ and coding region of selected vertebrate genes and estimates the size of the gene
aBased on the 5′ longest transcript
bComplete open reading frame within the genomic sequence but intron within the very N terminus cannot be ruled out because of lack of mRNA information