Table 3.
Sequence conservation of P2Y12-like GPCR
| Receptor | Ka/Ks | Pi |
|---|---|---|
| (mean ± SD) | (mean ± SD) | |
| P2Y12 | 0.049 ± 0.018 | 0.145 ± 0.022 |
| P2Y13 | 0.141 ± 0.070 | 0.187 ± 0.023 |
| P2Y14 | 0.103 ± 0.036 | 0.190 ± 0.024 |
| GPR87 | 0.046 ± 0.027 | 0.134 ± 0.018 |
| GPR171 | 0.078 ± 0.012 | 0.158 ± 0.021 |
| GPR34 | 0.082 ± 0.037 | 0.137 ± 0.026 |
| GPR82 | 0.202 ± 0.049 | 0.167 ± 0.034 |
To compare the sequence conservation of P2Y12-like receptors, ortholog sequences [relative positions 1.48 (in TMD1) to 7.68 (in C-term)] from 18 species were aligned and DNA polymorphism analyses were performed using DnaSP (version 4.1). The Ka/Ks ratio is calculated from the number of nonsynonymous substitutions per nonsynonymous site (Ka) and the number of synonymous substitutions per synonymous site (Ks) for any pair of sequences. The nucleotide diversity (Pi) is the average number of nucleotide differences per site between two sequences. The orthologs of the following species were analyzed because their genome contained all members of the P2Y12-like group: Bos taurus, Equus caballus, Canis familiaris, Pteropus vampyrus, Ornithorhynchus anatinus, Monodelphis domestica, Callithrix jacchus, Pan troglodytes, Homo sapiens, Macaca mulatta, Microcebus murinus, Pongo pygmaeus, Tarsius syrichta, Cavia porcellus, Mus musculus, Rattus norvegicus, Tursiops truncates, Gallus gallus