Table 1.
Summary of classification test results using standard procedures and kits
| Test | Contaminant 1 | Contaminant 2 | Contaminant 3 |
|---|---|---|---|
| Morphology of colonies | |||
| Form | Round | Round | Round |
| Diameter | 1–2 mm | 1 mm | 1 mm |
| Color | Grey | Beige | White |
| Brim | Smooth | Smooth | Smooth |
| Surface | Shiny | Shiny | Shiny |
| Cross section | Raised | Raised | Raised |
| Gram stain | Negative | Negative | Negative |
| Biochemistry | |||
| Catalase | + | + | + |
| Oxidase | + | + | – |
| O/F test | –/– | –/– | –/– |
| Motility | + | + | – |
| Lysine/BBL Ox/Ferm Tube II | – | + | n.d. |
| Result | Achromobacter spp. or Pseudomonas spp. | Achromobacter spp. | Acinetobacter spp. |
| Api 20 NE | |||
| NO3, nitrate reduction | + | + | – |
| GLU, glucose | + | – | – |
| GNT, gluconate | + | + | – |
| CAP, caprate | + | – | + |
| ADI, adipate | + | + | – |
| MLT, maltose | + | + | + |
| CIT, citrate | + | + | – |
| PAC, phenylacetate | + | + | + |
| OX, oxidase | + | + | – |
| Result | A. xylosoxidans (94.5%) | A. denitrificans (82.2%) | A. lwoffii (98.1%) |
| VITEK 2 | |||
| N-LGGH, γ-L-glutamyl (nutrient assimilation, NA) | + | + | n.d. |
| P-BPHO, bis(p-nitrophenyl)phosphate | + | – | n.d. |
| C-CIT, citrate | – | + | n.d. |
| C-KGA, α-acetoglutaryc acid | + | + | n.d. |
| N-LPROT, L-proline-NA | + | – | n.d. |
| Result | Achromobacter spp. (90%) | Achromobacter spp. (90%) | n.d. |
n.d. not determined