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Immunoblot analyses of purinoceptor (a) and ecto-enzyme (b) expression by transfected HEK cells. Untransfected (u) or HEK cells transfected with mouse (m) or human (h) purinoceptors or ecto-enzymes were solubilized 24 h post-transfection with SDS-PAGE sample buffer. Proteins in cell lysates were size-fractionated by SDS-PAGE and subjected to immunoblot analyses using the indicated antisera. Bound antibodies were detected with peroxidase-conjugated secondary antibody and the ECL system
