Fig. 5.

Immunoprecipitation of purinoceptors by antibodies raised via genetic immunization. Untransfected (u) and transfected (t) HEK cells were solubilized 24 h post-transfection with PBS/1% Triton X-100. Purinoceptors were immunoprecipitated from cell lysates by incubation with the indicated antisera bound to Protein G Sepharose. Precipitates were subjected to immunoblot analysis as in Fig. 4, using the indicated antipeptide antisera for detection