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. Author manuscript; available in PMC: 2007 Nov 10.
Published in final edited form as: J Cell Biochem. 2007 Sep 1;102(1):196–210. doi: 10.1002/jcb.21287

Fig. 5.

Fig. 5

Mitochondrial-targeted active Akt protects SH-SY5Y cells from staurosporine-induced caspase activation. SH-SY5Y cells infected with the designated adenoviral constructs were serum starved overnight and incubated in the absence (control) or presence (staurosporine) of 0.5 μM staurosporine for 2 h (caspase-3 assay and PARP) or 0–2 h (caspase-9 activity). A: Caspase-3 activity. The presence of Ad-HA-Akt (DD) attenuated caspase-3 activation in response to staurosporine, with a significantly greater decrease being observed with Ad-Mito-HA-Akt (DD). The data are presented as mean ± SEM, n = 7 independent experiments; *, P < 0.05; ***, P < 0.0005 (one-way ANOVA). B: Representative blots showing the presence of cleaved PARP, cleaved caspase-3 and Akt. C: Caspase-9 activity. The presence of Ad-HA-Akt (DD) attenuated caspase-9 activation in response to staurosporine, with a significantly greater decrease being observed with Ad-Mito-HA-Akt (DD). The data are presented as mean ± SEM, n = 10 independent experiments; *, P < 0.05; †, P < 0.001; **, P <0.005 (two-tailed, paired Student’s t-test).