Figure 2.

(A) mRNA for gelatinase A as shown by Northern blot analysis and quantitated by normalizing to 28s RNA. (B) Time course of appearance of 62-kDa gelatinase A in cell extracts. Western immunoblots of cell extracts using affinity-purified gelatinase A antibody were performed as described. The arrow indicates 62-kDa active enzyme. Lanes: A, monolayer control extract 24 h in ITS serum-free medium; B, monolayer control extract 70 h in ITS serum-free medium; C, 0 time extract of trypsinized cells; D, 20-min lattice extract; E, 1-h lattice extract; F, 3-h lattice extract; G, 5-h lattice extract; H, 24-h lattice extract; and I, 48-h lattice extract.