Table 2.
Fraction | Marker enzymes
|
||
---|---|---|---|
Plasma membrane, 5′-Nucleotidase, μMol Pi/30 min | Endoplasmic reticulum, NADH cytochrome c reductase, μmol NADH/30 min | Golgi membranes, UDP galactosyl transferase, nmol galactosyl × 105/16 h | |
Crude plasma membranes | 0.216 | 46 | 9.8 |
Percoll-purified plasma membranes | 0.487 | 0 | 1.3 |
Golgi-enriched membranes | 0.299 | 28 | 12.8 |
Cytosol | 0.085 | 0 | 9.3 |