Figure 2.

Concentration-dependent effects of InsP₆ on insulin secretion in electropermeabilized cells in the presence of either 30 nM (A) or 10 μM (B) Ca²⁺ and on free Ca²⁺-concentration in the permeabilization buffer containing either ≈30 nM (C) or ≈10 μM (D) Ca²⁺. For measurements of insulin secretion, data represent mean ± SEM for 18 observations from three separate experiments. ∗∗∗, P < 0.001, relative to insulin secretion without InsP₆. For measurements of Ca²⁺, representative experiments of three are presented.