Figure 4.

Increase of h-sgk expression following hypertonic exposure in the absence and presence of cycloheximide. HepG2 cells were incubated with (+) or without (−) cycloheximide (10 μg/ml) 2 hr prior to transfer in hypertonic BME with (+) or without (−) cycloheximide for an additional 2 hr. Each slot of the gel was loaded two times with 20 μg of total cytoplasmic RNA with an interval of 15 min between the two loadings. The lower and the upper rows represent the expression of h-sgk under isotonic (iso, 290 mosmol/liter) or hypertonic conditions (hyper, 390 mosmol/liter), respectively.