Figure 2.

Recombinant karyopherin β3. (A) Full-length (lane 1) and C-terminal 183 amino acids (lane 2) of karyopherin β3 were subjected to SDS/PAGE on a 5–20% acrylamide gradient gel and stained with Coomassie blue. Places of molecular mass markers are indicated to the left. (B) Fifty nanograms of recombinant karyopherin β3 (lane 1) and 40 μg of HeLa cytosol S100 fraction (lane 2) were electrophoresed, transferred to nitrocellulose, and immunoblotted with anti-karyopherin β3 antibody 384AP, and the signal was detected by chemiluminescence.