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. 1997 Apr 29;94(9):4451–4456. doi: 10.1073/pnas.94.9.4451

Figure 6.

Figure 6

Karyopherin β3 binds to ribosomal proteins in an overlay assay. Ribosomal proteins were separated by reverse-phase HPLC, electrophoresed, transferred to nitrocellulose membrane, stained with amido black (Left), and subjected to overlay assay with recombinant karyopherin β3 (Right). Fractions 6–16 are shown; the remaining fractions were negative by overlay assay (not shown). Small arrows indicate the positive bands on the amido black-stained gel.