Figure 1.
A fraction of cytoplasmic matrix and membrane protein from regenerated flagella contains a 17S protein complex. (a) Sedimentation profile of 35S-labeled Nonidet P-40-soluble proteins extracted from flagella regenerated from wild-type Chlamydomonas cells. Vertical lines indicate the position of standards that were sedimented in a parallel experiment; cpm refers to 10-μl aliquots of 170-μl fractions. (b) Electrophoretograms of proteins contained in sucrose gradient fractions 4–18. Molecular weight standards are indicated on the left. Lines between lanes 10 and 11 indicate the presence of components 1–13 of the 17S complex.