Figure 3.

Western blots by antibodies specific for p28. (a) Gradient fractions resolving proteins of the cytoplasmic matrix from regenerated flagella. The polypeptide content of these fractions is that shown in Fig. 1b. (b) Gradient fractions resolving inner and outer dynein arms that were extracted from regenerated axonemes by 0.6 M NaCl and 1 mM ATP-Mg (18). This extract was prepared from axonemes of the same flagella that provided the cytoplasmic matrix. Furthermore, it was brought to the same ionic and detergent concentrations of the cytoplasmic matrix.