Figure 5.

The 17S complex requires the activity of KHP1 to be present in flagella. (a and b) Electrophoretograms of proteins contained in sucrose gradient fractions 6–17 resolving proteins extracted from fla10 flagella by ultrasound. Proteins from fla10 cells exposed at 21°C (a) and 32°C (b). Molecular weight standards are indicated on the left. Lines between lanes 10 and 11 of a indicate components 1–13 of the 17S complex. Dots between lanes 15 and 16 indicate inner dynein arm subunits actin, p28, and caltractin/centrin, respectively.