Figure 6.

Subunits of the 17S complex turn over in the cytoplasmic matrix more rapidly than inner dynein arm subunits actin, p28, and caltractin/centrin in the axonemes. Wild-type cells were cultured in medium containing reduced amount of sulfates and pulse labeled with [³⁵S]H₂SO₄ for 30 min at 21°C. Matrix proteins were isolated by ultrasound. Electrophoretogram of pulse labeled proteins contained in sucrose gradient fractions 6–17. Molecular weight standards are indicated on the left. Lines between lanes 10 and 11 indicate the presence of components 1–5, 7, 8, 10, and 11 of the 17S complex.