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Graphs showing the effect of MAP60 on the cold and cold/calcium stability of microtubules. Rhodamine-labeled microtubules (40 μM) were assembled in the presence of 0.1 μM MAP60 followed by an incubation on ice for 45 min. (A) Control before cold incubation; (B) microtubules after 45 min on ice; and (C) microtubules after 45 min on ice in the presence of 5 mM CaCl2.
