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. 1996 Nov;74(9):1445–1451. doi: 10.1038/bjc.1996.563

Immunohistochemical determination of oestrogen receptor: comparison of different methods of assessment of staining and correlation with clinical outcome of breast cancer patients.

D M Barnes 1, W H Harris 1, P Smith 1, R R Millis 1, R D Rubens 1
PMCID: PMC2074773  PMID: 8912543

Abstract

Immunohistochemical staining for oestrogen receptor (ER) has been carried out using antibody ER ID5 on 170 women who received first-line tamoxifen treatment for evaluable metastatic breast cancer. ER status had been determined some years previously, using a ligand-binding cytosol assay. The adequacy of the tissue used for the cytosol assay was always checked by histology on an adjacent block and was deemed to be typical of the tumour overall as was the block used for immunohistochemistry. Six different methods were used to assess the degree of staining and comparisons were made to determine which method gave the most clinically relevant results. Clinical outcome was assessed both in terms of duration of response to tamoxifen determined by log-rank analysis and type of response using the chi-squared test. The ER immunohistochemical assay gave superior results compared with the cytosol assay, with all of the subjective methods of assessment of staining giving statistically significant correlations with clinical outcome. The additional contribution of progesterone receptor (PR) staining with antibody NCL PGR was also studied.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen J., Thorpe S. M., King W. J., Rose C., Christensen I., Rasmussen B. B., Poulsen H. S. The prognostic value of immunohistochemical estrogen receptor analysis in paraffin-embedded and frozen sections versus that of steroid-binding assays. Eur J Cancer. 1990 Apr;26(4):442–449. doi: 10.1016/0277-5379(90)90013-j. [DOI] [PubMed] [Google Scholar]
  2. Bobrow L. G., Happerfield L. C., Gregory W. M., Springall R. D., Millis R. R. The classification of ductal carcinoma in situ and its association with biological markers. Semin Diagn Pathol. 1994 Aug;11(3):199–207. [PubMed] [Google Scholar]
  3. Elston C. W. The assessment of histological differentiation in breast cancer. Aust N Z J Surg. 1984 Feb;54(1):11–15. doi: 10.1111/j.1445-2197.1984.tb06677.x. [DOI] [PubMed] [Google Scholar]
  4. Goulding H., Pinder S., Cannon P., Pearson D., Nicholson R., Snead D., Bell J., Elston C. W., Robertson J. F., Blamey R. W. A new immunohistochemical antibody for the assessment of estrogen receptor status on routine formalin-fixed tissue samples. Hum Pathol. 1995 Mar;26(3):291–294. doi: 10.1016/0046-8177(95)90060-8. [DOI] [PubMed] [Google Scholar]
  5. Greene G. L., Nolan C., Engler J. P., Jensen E. V. Monoclonal antibodies to human estrogen receptor. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5115–5119. doi: 10.1073/pnas.77.9.5115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hayward J. L., Carbone P. P., Heuson J. C., Kumaoka S., Segaloff A., Rubens R. D. Assessment of response to therapy in advanced breast cancer: a project of the Programme on Clinical Oncology of the International Union Against Cancer, Geneva, Switzerland. Cancer. 1977 Mar;39(3):1289–1294. doi: 10.1002/1097-0142(197703)39:3<1289::aid-cncr2820390340>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  7. Hayward J. L., Meakin J. W., Stewart H. J. Assessment of response and recurrence in breast cancer. Semin Oncol. 1978 Dec;5(4):445–449. [PubMed] [Google Scholar]
  8. Horwitz K. B., McGuire W. L. Predicting response to endocrine therapy in human breast cancer: a hypothesis. Science. 1975 Aug 29;189(4204):726–727. doi: 10.1126/science.168640. [DOI] [PubMed] [Google Scholar]
  9. King W. J., DeSombre E. R., Jensen E. V., Greene G. L. Comparison of immunocytochemical and steroid-binding assays for estrogen receptor in human breast tumors. Cancer Res. 1985 Jan;45(1):293–304. [PubMed] [Google Scholar]
  10. Kinsel L. B., Szabo E., Greene G. L., Konrath J., Leight G. S., McCarty K. S., Jr Immunocytochemical analysis of estrogen receptors as a predictor of prognosis in breast cancer patients: comparison with quantitative biochemical methods. Cancer Res. 1989 Feb 15;49(4):1052–1056. [PubMed] [Google Scholar]
  11. Press M. F., Greene G. L. Localization of progesterone receptor with monoclonal antibodies to the human progestin receptor. Endocrinology. 1988 Mar;122(3):1165–1175. doi: 10.1210/endo-122-3-1165. [DOI] [PubMed] [Google Scholar]
  12. Reiner A., Neumeister B., Spona J., Reiner G., Schemper M., Jakesz R. Immunocytochemical localization of estrogen and progesterone receptor and prognosis in human primary breast cancer. Cancer Res. 1990 Nov 1;50(21):7057–7061. [PubMed] [Google Scholar]
  13. Saccani Jotti G., Johnston S. R., Salter J., Detre S., Dowsett M. Comparison of new immunohistochemical assay for oestrogen receptor in paraffin wax embedded breast carcinoma tissue with quantitative enzyme immunoassay. J Clin Pathol. 1994 Oct;47(10):900–905. doi: 10.1136/jcp.47.10.900. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stewart J., King R., Hayward J., Rubens R. Estrogen and progesterone receptors: correlation of response rates, site and timing of receptor analysis. Breast Cancer Res Treat. 1982;2(3):243–250. doi: 10.1007/BF01806937. [DOI] [PubMed] [Google Scholar]
  15. Taylor C. R., Shi S. R., Chaiwun B., Young L., Imam S. A., Cote R. J. Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by antigen retrieval techniques. Hum Pathol. 1994 Mar;25(3):263–270. doi: 10.1016/0046-8177(94)90198-8. [DOI] [PubMed] [Google Scholar]
  16. Underwood J. C. Oestrogen receptors in human breast cancer: review of histopathological correlations and critique of histochemical methods. Diagn Histopathol. 1983 Jan-Mar;6(1):1–22. [PubMed] [Google Scholar]
  17. al Saati T., Clamens S., Cohen-Knafo E., Faye J. C., Prats H., Coindre J. M., Wafflart J., Caverivière P., Bayard F., Delsol G. Production of monoclonal antibodies to human estrogen-receptor protein (ER) using recombinant ER (RER). Int J Cancer. 1993 Oct 21;55(4):651–654. doi: 10.1002/ijc.2910550423. [DOI] [PubMed] [Google Scholar]

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