Figure 1.

G-substrate mRNA levels in mouse and human DA neurons. b–f, DA neurons were labeled using the quick TH staining method in mouse, as described previously (Chung et al., 2005), and human midbrain. d–f, DA neurons were then collected using laser capture microdissection. g, Quantitative PCR results demonstrated that A10 DA neurons showed higher levels of G-substrate mRNA than A9 DA neurons in both rodent and human. Data are shown as mRNA ratios of A10 over A9 DA neurons ± SEM (n = 3 for mouse, n = 4 for male human, and n = 3 for female human; *p < 0.05, two-tailed t test). SNc, Substantia nigra pars compacta.