Figure 6.

Knockdown of endogenous Akt decreases G-substrate-induced protection against 6-OHDA. a, Western blot analysis confirmed that Akt siRNA application in BE(2)-M17 cells substantially reduced the Akt protein levels. b, Cytotoxicity was measured in the presence or absence of 50 μm 6-OHDA treatment after applying control or Akt siRNA to both control and G-substrate-expressing cells. Data are shown as means ± SEM of four independent experiments (*p < 0.001, one-way ANOVA, Tukey's test). These data were used to generate the percentage decrease in cytotoxicity afforded by G-substrate [(control − G-substrate)/control] in the presence of Akt (55.6 ± 8.1%) or with Akt knocked down (23.1 ± 4.9%; c). Data are shown as means ± SEM of four independent experiments (**p < 0.05, two-tailed t test).