Figure 4.

Definitive erythroid cells express LacZ in pITintIEintLacZ transgenic mice. Data from line 226 (see also Table 1) are shown. (a and b) Histological examination of a 12.5-dpc fetal liver, where prevalent X-Gal expression was found. (c) anti-GATA-1 antibody staining of 12.5-dpc fetal liver. Note that the number of X-Gal-positive cells is comparable to that of the antibody staining-positive cells in the fetal liver. (d) X-Gal staining of adult mouse spleen. The expression of LacZ persists in the adult mouse spleen, where many cells in the red pulp show X-Gal staining. (a and d, ×25; c, ×50; and b, ×100.)