Table 1.
Expression of LacZ in 8.5-dpc yolk sac and 12.5-dpc fetal liver cells of transgenic mouse lines
| Constructs | Line | 8.5-dpc yolk sac (blue blood island) | 12.5-dpc fetal liver, % |
|---|---|---|---|
| a. | |||
| IE3.9LacZ | 404 | >100 | 0.9 |
| 932 | >100 | 1.0 | |
| 927 | 0 | 0 | |
| 1 | >100 | ND | |
| b. | |||
| IE2.6LacZ | 547 | 0 | 0 |
| 2 | 0 | ND | |
| 5 | 0 | ND | |
| c. | |||
| IE3.9intLacZ | 508 | >100 | 24 |
| 515 | >100 | 11 | |
| 455 | >100 | 41 | |
| 467 | >100 | 0.8 | |
| d. | |||
| IE2.6intLacZ | 855 | 0 | 0 |
| 973 | 0 | 0 | |
| 803 | 0 | 0 | |
| e. | |||
| ITintIELacZ | 707 | >100 | 0.8 |
| 716 | >100 | 0.8 | |
| 12 | 0 | ND | |
| f. | |||
| ITintIEintLacZ | 226 | >100 | 42 |
| 114 | 58 | 23 | |
| 9 | ND | 20 |
Numbers of blue blood islands containing X-Gal-positive cells were counted in the 8.5-dpc yolk sacs. Total number of blue blood islands in one typical yolk sac is shown. We also counted 1000 cells in 12.5-dpc fetal liver sections from various transgenic embryos. The percentage of X-Gal-positive blue cells is shown. Lines 1, 2, 5, 9, and 12 were analyzed as F0 embryos. Although a few LacZ-positive cells were observed in the 12.5-dpc fetal liver section of pIE3.9LacZ and pITintIELacZ transgenic mice (see Figs. 3f and 5b), the same level expression also was observed in nontransgenic embryos. ND, not determined.